Biotechnology Class 12 Syllabus
Exam Structure
| Units | Topics | Marks |
| Unit V | Protein and Gene Manipulation | 40 |
| Unit VI | Cell Culture and Genetic Manipulation | 30 |
| Practicals | 30 | |
| Total | 100 |
Unit V: Protein and Gene Manipulation
Chapter-1: Recombinant DNA Technology
Introduction; Tool of rDNA Technology; Marketing Recombinant DNA; Introduction of Recombinant DNA into Host Cells; Identification of Recombinants; DNA Library; DNA Probes; Hybridization Techniques; Polymerase Chain Reaction (PCR); DNA Secquencing; Site-directed Mutagenesis
Chapter-2: Protein Structure and Engineering
Introduction to the World of Proteins; 3-D Shape of Proteins; Structure-Function Relationship in Proteins Purification of Proteins; Characterization of Proteins; Protein based Products; Designing Proteins (protein engineering)
Chapter-3: Genomics and Bioinformatics
Introduction; Genome Sequencing Projects; Gene Prediction and Counting; Genome Similarity, SNPs and Comparative Genomics; Functional Genomics; Proteomics; History of Bioinformatics; Sequences and Nomenclature; Information Sources; Analysis using Bioinformatics Tools
Unit VI: Cell Culture and Genetic Manipulation
Chapter-1: Microbial Culture and Applications
Introduction, Microbial culture techniques, Measurement and kinetics of microbial growth, Scale up of microbial process, Isolation of microbial products, Strain isolation and improvement, Applications of microbial culture technology, Biosafety issues in microbial technology
Chapter-2: Plant Cell Culture and Applications
Introduction; Cell and Tissue Culture Techniques; Applications of Cell and Tissue Culture; Gene Transfer Methods in Plants; Transgenic Plants with Beneficial Traits; Biosafety in Plant Genetic Engineering
Chapter-3: Animal Cell Culture and Applications
Introduction, Animal cell culture techniques, Characterisation of cell lines, Methods of gene delivery into cells, Scale-up of animal culture process, Applications of animal cell culture, Stem cell technology, Tissue engineering
Practicals
- Use of special equipment in biotechnology experiments.
- Isolation of bacterial plasmid DNA
- Detection of DNA by gel electrophoreses
- Isoloation of Genomic DNA (CTAB method)
- Estimation of DNA
- Bacterial transformation using any plasmid
- Restriction digestion of plasmid DNA & its analysis by gel electrophoreses
- Isolation of bacterial grom curd & staining of bacteria
- Cell viability assay
- Data retrieval and data base search using internet site NCBI and download a DNA and protein sequence from internet, analyse it and comment on it
- Reading of a DNA sequencing gel to arrive at the sequence
- Project work
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